Changes of proteomic spectra of total flavones of Hippophae rhamnoides on myocardial protection / 中国中药杂志
Zhongguo Zhong Yao Za Zhi
; (24): 1060-1063, 2008.
Article
en Zh
| WPRIM
| ID: wpr-295408
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the mechanisms of the anti-myocardial ischemia of total flavones of Hippophae rhamnoides (TFH) at the level of proteome.</p><p><b>METHOD</b>Surface enhanced laser desorption/ionization (SELDI) mass spectrometry with protein chip IMAC3, SAX2 and NP20 was performed to compare the differentially expressed protein in myocardial ischemia in the TFH-treated groups with the 0.9% sodium chloride groups. Protein chips were examined in PBS II - C protein chip reader (ciphergen ciosystem inc) and the protein profiling was analyzed by Proteinchip Software 3. 0. 2.</p><p><b>RESULT</b>The revealed six peaks had significant difference between the TFH-treated groups and the control groups, one of which were up-regulated in the TFH-treated groups, and the other were down-regulated. And in these six distinct proteins, there were four proteins on the IMAC3 chips and one protein on the SAX2 chips.</p><p><b>CONCLUSION</b>The TFH could prevent the myocardium from ischemia via regulating expression of different proteins.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Farmacología
/
Medicamentos Herbarios Chinos
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Distribución Aleatoria
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Química
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Ratas Wistar
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Isquemia Miocárdica
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Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
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Hippophae
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Proteómica
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Flavonas
Límite:
Animals
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Año:
2008
Tipo del documento:
Article