Determination in vitro of rat plasma protein binding rate of sinomenine by using microdialysis method / 药学学报
Yao Xue Xue Bao
; (12): 909-912, 2006.
Article
en Zh
| WPRIM
| ID: wpr-294915
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>AIM</b>To determine in vitro the rat plasma protein binding rate by using microdialysis method.</p><p><b>METHODS</b>The binding rate was determined by using microdialysis probe as sampling tools and zero-net flux method as calibrating method. The regression equation was made by the difference of concentrations between the dialysis sample and the perfusate. The x-intercept of regression equation was the free drug concentration (Cf). The plasma protein binding rate was calculated by using the following equation: f = ( C0 - Cf)/C0.</p><p><b>RESULT</b>The binding rate was kept relatively stable in the studied concentration range.</p><p><b>CONCLUSION</b>It is feasible that the plasma protein binding rate can be determined by using microdialysis method.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Plantas Medicinales
/
Unión Proteica
/
Proteínas Sanguíneas
/
Química
/
Análisis de Regresión
/
Cromatografía Líquida de Alta Presión
/
Ratas Sprague-Dawley
/
Microdiálisis
/
Sinomenium
/
Metabolismo
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
Zh
Revista:
Yao Xue Xue Bao
Año:
2006
Tipo del documento:
Article