Analysis of mitochondrial DNA D-loop region mutation and its significance in human oncocytoma / 中华肿瘤杂志
Zhonghua zhong liu za zhi
; (12): 767-770, 2010.
Article
en Zh
| WPRIM
| ID: wpr-293486
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the mutation in mitochondrial DNA displacement-loop (mtDNA D-loop) region in oncocytoma and its relationship with tumorigenesis and tumor development.</p><p><b>METHODS</b>The mtDNA D-Loop region of 20 thyroid or renal oncocytomas and the adjacent normal tissues were amplified by PCR, and then sequenced. Five human fetal renal tissues were collected as matched controls.</p><p><b>RESULTS</b>Among the 20 oncocytomas, 21 mutations which focused on hypervariable region I (HVI) were found in 7 tumor tissues and 1 normal tissue with the mutation rates of 35% and 5%, respectively. At the same time, 191 polymorphisms were found in the 20 cases.</p><p><b>CONCLUSION</b>mtDNA D-loop region, especially HV I, is the mutational hotspot of oncocytomas, which may be closely related with mtDNA duplicating rate and the function of mitochondria.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Polimorfismo Genético
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ADN Mitocondrial
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Neoplasias de la Tiroides
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Análisis Mutacional de ADN
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Secuencia de Bases
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Adenoma Oxifílico
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Genética
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Neoplasias Renales
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Mitocondrias
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Mutación
Límite:
Female
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Humans
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Male
Idioma:
Zh
Revista:
Zhonghua zhong liu za zhi
Año:
2010
Tipo del documento:
Article