Analysis of methylation status of the promoter of mdr1 gene in K562 and K562/DNR cells / 中华血液学杂志
Chinese Journal of Hematology
; (12): 293-295, 2004.
Article
en Zh
| WPRIM
| ID: wpr-291445
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the methylation patterns of mdr1 gene promotor in both K562 cell and K562/DNR cells and the relationship between promotor methylation and P-gp expression.</p><p><b>METHODS</b>mdr1 gene expression was analyzed by flow cytometry and RT-PCR, methylation status of the promotor of mdr1 gene by bisulfite-sequencing (including two GC-box).</p><p><b>RESULTS</b>mdr1 gene was found methylated at GC-box and not expressed in K562 cells, but unmethylated and expressed respectively in K562/DNR cells.</p><p><b>CONCLUSION</b>The methylation patterns of mdr1 gene promotor in K562/DNR and K562 cells were different. mdr1 gene silencing was associated with the promotor methylation.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Secuencia de Bases
/
Regiones Promotoras Genéticas
/
Análisis de Secuencia de ADN
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Miembro 1 de la Subfamilia B de Casetes de Unión a ATP
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Metilación de ADN
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Células K562
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Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Silenciador del Gen
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Citometría de Flujo
/
Genética
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Hematology
Año:
2004
Tipo del documento:
Article