A combined assay of multiplex RT-PCR and karyotypic analysis in childhood acute lymphoblastic leukemia / 中华血液学杂志
Chinese Journal of Hematology
; (12): 413-416, 2004.
Article
en Zh
| WPRIM
| ID: wpr-291404
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the value of combination assay of multiplex RT-PCR and karyotypic analysis in the diagnosis and classification of childhood acute lymphoblastic leukemia (ALL).</p><p><b>METHODS</b>Fifty cases of childhood ALL patients were studied by multiplex RT-PCR in combination with R or G banding karyotype analysis.</p><p><b>RESULTS</b>Of the 50 childhood ALL patients, 18 (36.0%) carried 11 types of fusion genes including E2A/PBX1, TEL/AML1, TLS/ERG, MLL/AF4, MLL/AF9, MLL/AF10, MLL/AFX, MLL/AF6, MLL/ELL, TAL1D, and HOX11, revealed by multiplex RT-PCR, and in 48 cases, 24 (57.1%) had chromosome abnormalities. Among the latter, numeral chromosome abnormalities and chromosome deletions accounted for 75.0% (18/24), while translocations 25.0% (6/24). The multiplex RT-PCR in combination with chromosome analysis could detect genetic abnormalities in 70% (35/50) of childhood ALL.</p><p><b>CONCLUSIONS</b>Multiplex RT-PCR combined with chromosome analysis can enhance the detection rate of genetic abnormalities in childhood ALL. It provides reliable evidence for the diagnosis, classification and prognosis.</p>
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Base de datos:
WPRIM
Asunto principal:
Proteínas de Fusión Oncogénica
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Reproducibilidad de los Resultados
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Aberraciones Cromosómicas
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Sensibilidad y Especificidad
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Clasificación
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Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
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Diagnóstico
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Leucemia-Linfoma Linfoblástico de Células Precursoras
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Genética
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Cariotipificación
Tipo de estudio:
Diagnostic_studies
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Prognostic_studies
Límite:
Adolescent
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Child
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Child, preschool
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Female
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Humans
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Infant
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Male
Idioma:
Zh
Revista:
Chinese Journal of Hematology
Año:
2004
Tipo del documento:
Article