Exogenous rhCNTF inhibits myoblast differentiation of skeletal muscle of adult human in vitro / 生理学报
Acta Physiologica Sinica
; (6): 464-468, 2003.
Article
en Zh
| WPRIM
| ID: wpr-290942
Biblioteca responsable:
WPRO
ABSTRACT
To determine the role of recombinant human ciliary neurotrophic factor (rhCNTF) in myogenesis, we observed the effects of rhCNTF (0 10 ng/ml) on myoblast differentiation of adult human in vitro. The results showed that compared with the control group, the groups of 2.5 10 ng/ml rhCNTF treatment significantly inhibited myoblast differentiation (P<0.01), and the inhibition was dose-dependent and reversible. Western blots also indicated that compared with the control group, the expression of myogenin and p21, markers of myoblast differentiation phase, was significantly reduced (P<0.01), while Myf5 and desmin, markers of myoblast proliferative phase, significantly increased (P<0.01) in the groups of 2.5 10 ng/ml rhCNTF treatment. These findings demonstrate that exogenous rhCNTF can reversibly inhibit differentiation but permits proliferation of adult human myoblasts in vitro.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Farmacología
/
Proteínas Recombinantes
/
Diferenciación Celular
/
Células Cultivadas
/
Músculo Esquelético
/
Factor Neurotrófico Ciliar
/
Biología Celular
/
Mioblastos
/
Proliferación Celular
Límite:
Adult
/
Humans
Idioma:
Zh
Revista:
Acta Physiologica Sinica
Año:
2003
Tipo del documento:
Article