Determination of ascitic bacterial 16S rRNA by quantitative PCR-microarray in the diagnosis of spontaneous bacterial peritonitis / 中华肝脏病杂志
Zhonghua ganzangbing zazhi
; Zhonghua ganzangbing zazhi;(12): 297-300, 2011.
Article
en Zh
| WPRIM
| ID: wpr-290612
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the significance of determining ascitic bacterial 16S rRNA by quantitative PCR combined with microarray (PCR-microarray) in the diagnosis of spontaneous bacterial peritonitis (SBP).</p><p><b>METHODS</b>Ascitic bacterial 16SrRNA was determined by real time fluorescent quantitative PCR-microarray in 76 cases of suspected SBP and 6 cases of non-infectious ascites with chronic liver diseases. The results were compared with ascitic bacterial culture simultaneously.</p><p><b>RESULTS</b>Of 76 ascitic samples, 17 were detected bacteria positive by PCR-microarray, including 8 Grams positive(G+) and 9 Grams negative(G-), which was higher than that by bacterial culture which had only 6 ascitic samples detected positive (all G-); the positive rates were 22.4% vs 7.9%, respectively (P < 0.01). The bacterial strains detected by both methods in 6 cases had a consistency with each other. No bacteria were detected in another 6 cases of non-infectious ascites with chronic liver diseases.</p><p><b>CONCLUSIONS</b>Determination of ascitic bacteria 16S rRNA by PCR-microarray has a higher specificity and sensitivity in the diagnosis of SBP as compared with the bacteria culture. Application of this novel method can not only accelerate SBP diagnosis but also stratify the different pathogens.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Peritonitis
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Infecciones Bacterianas
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Líquido Ascítico
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ARN Bacteriano
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ARN Ribosómico 16S
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Reacción en Cadena de la Polimerasa
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Análisis de Secuencia por Matrices de Oligonucleótidos
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Diagnóstico
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Cirrosis Hepática
/
Métodos
Tipo de estudio:
Diagnostic_studies
Límite:
Adult
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Aged
/
Female
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Humans
/
Male
Idioma:
Zh
Revista:
Zhonghua ganzangbing zazhi
Año:
2011
Tipo del documento:
Article