Inhibitory effect of SHP-1 gene transfer on the proliferation of breast cancer cell line MDA-MB-231 / 南方医科大学学报
Journal of Southern Medical University
; (12): 1024-1027, 2010.
Article
en Zh
| WPRIM
| ID: wpr-290003
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe SHP-1 protein expression in breast cancer cell line MDA-MB-231 before and after SHP-1 gene transfer and its effect on the proliferation of MDA-MB-231 cells.</p><p><b>METHODS</b>The eukaryotic expression vector pEGFP-C3-SHP-1 was constructed and transfected into breast cancer cell line MDA-MB-231 via Lipofectamine 2000, and the positive clones were selected using G418. SHP-1 expression in MDA-MB-231 cells was detected with immunocytochemistry and Western blotting, and the cell growth curve was observed using MTT assay.</p><p><b>RESULTS</b>SHP-1 was highly expressed in transfected MDA-MB-231 cells, whose proliferation was significantly inhibited (P<0.05).</p><p><b>CONCLUSION</b>SHP-1 gene transfer into MDA-MB-231 cells results in inhibition of the cell proliferation.</p>
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Base de datos:
WPRIM
Asunto principal:
Patología
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Neoplasias de la Mama
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Técnicas de Transferencia de Gen
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Línea Celular Tumoral
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Proliferación Celular
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Proteína Tirosina Fosfatasa no Receptora Tipo 6
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Genética
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Metabolismo
Límite:
Female
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Humans
Idioma:
Zh
Revista:
Journal of Southern Medical University
Año:
2010
Tipo del documento:
Article