An experimental study on the influence of inhibition of postburn stress on inflammatory reaction in severely scalded rats / 中华烧伤杂志
Chinese Journal of Burns
; (6): 268-271, 2002.
Article
en Zh
| WPRIM
| ID: wpr-289199
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the influence of hibernation drugs on postburn stress and inflammatory reaction in severely scalded rats.</p><p><b>METHODS</b>Sprague-Dawley rats inflicted with 30% TBSA deep partial thickness scalding were employed as the model. The rats were divided into A (scalding with immediate resuscitation), B (scalding with immediate resuscitation and lytic cocktail), C (scalding with delayed resuscitation), D (scalding with delayed resuscitation and lytic cocktail) and E (sham injury) groups. The rat plasma levels of NE (norepinephrine), E (epinephrine) and DA (dopamine) were determined by HPLC (high performance liquid chromatography) at 3, 6, 12, 24 and 48 postburn hours (PBHs), and the plasma IL-1alpha and PGE(2) levels were detected by ELISA (enzyme-linked immunosorbent assay) and RIA (radioimmunoassay) methods. The NF-kappaB activity in PBMCs (peripheral blood mononuclear cells) was determined by laser scanning confocal microscope.</p><p><b>RESULTS</b>The plasma NE and E levels reached summit at 6 PBH, while those in B group were lowest. But the plasma DA level was similar among all groups at all time points. The plasma IL-1alpha and PGE(2) levels increased continuously, however, the levels were were lower in B than A groups at the same time points, and also that of D were lower than C groups. At the same time points, the levels in B group were lower than those in D group. The NF-kappaB was located in the cytoplasma of PBMCs in E group and in the nucleus in A group at 6 PBH. Furthermore, the NF-kappaB was concentrated more in the cytoplasm than that in the nucleus in B group, while it was more concentrated in the nucleus in C and D groups.</p><p><b>CONCLUSION</b>The secretion of stress hormones could be attenuated by hibernation drugs. The plasma cytokine levels and the nuclear translocation of NF-kappaB in PBMCs could also be modulated by the drugs.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Estrés Fisiológico
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Sangre
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Dopamina
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Norepinefrina
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FN-kappa B
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Interleucina-1
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Ratas Sprague-Dawley
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Inflamación
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Metabolismo
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Burns
Año:
2002
Tipo del documento:
Article