Difference in effect between asthma-based mouse model and Stemona tuberosa extracts / 中国中药杂志
Zhongguo Zhong Yao Za Zhi
; (24): 4084-4087, 2013.
Article
en Zh
| WPRIM
| ID: wpr-287634
Biblioteca responsable:
WPRO
ABSTRACT
In this study, OVA-induced asthma mice was taken as the model, and orally administered with different concentration of ethanol extracts of crude and processed Stemona tuberosa, in order to determine the cytokine level released from Th1 and Th2 in splenocytes. RT-PCR was carried out to determine the genetic expression of T-bet/GATA-3 in lung, and compare the differentiation between ethanol extracts of crude and processed S. tuberosa in therapeutic effect on asthma in mice. According to the results, compared with the crude samples, processed samples significantly increased the levels of inflammatory factor INF-gamma (P < 0.05) and decreased IL-5 (P < 0.05) in splenocytes. According to the RT-PCR results, the administration of processed samples could increase the ratio of T-bet/GATA-3 (P < 0.05). The experiment showed that ethanol extracts of both crude and processed S. tuberosa could treat asthma by regulating Th1/Th2 ratio, but processed samples showed more notable effect. This indicated that crude and processed S. tuberosa had significant pharmacological difference. Therefore, it was more rational to apply processed S. tuberosa in clinical treatment of asthma and chronic cough, which layed a foundation for further revealing the processing mechanism of S. tuberosa.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Farmacología
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Asma
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Medicamentos Herbarios Chinos
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Química
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Regulación de la Expresión Génica
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Administración Oral
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Células Th2
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Células TH1
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Secreciones Corporales
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Proteínas de Dominio T Box
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Año:
2013
Tipo del documento:
Article