Construction of antisense recombinant adenoviral vector for c-myc and its antiproliferative effect on rat lymphocytes / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 220-223, 2005.
Article
en Zh
| WPRIM
| ID: wpr-287052
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>AIM</b>To observe the antiproliferative effect of antisense recombinant adenoviral vector for c-myc on rat thymus lymphocytes.</p><p><b>METHODS</b>Antisense and sense bacterial plasmids for c-myc were constructed. Bacterial plasmids and El detected adenoviral plasmid were cotransfected into 293 cells. Recombinant adenoviral vectors were obtained after cotransfection. The antiproliferative effects were assayed by MTS. The expression of c-myc mRNA was detected by RT-PCR.</p><p><b>RESULTS</b>The results showed that antisense recombinant adenoviral vector for c-myc could inhibit rat thymus lymphocytes proliferation. The expression of c-myc mRNA was decreased after antisense recombinant adenoviral vector for c-myc was transfected into cells.</p><p><b>CONCLUSION</b>Recombinant antisense adenoviral vector for c-myc could inhibit rat thymus lymphocytes proliferation.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Timo
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Linfocitos
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Línea Celular
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Adenoviridae
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Elementos sin Sentido (Genética)
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Genes myc
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Biología Celular
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Proliferación Celular
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Vectores Genéticos
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Genética
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Applied Physiology
Año:
2005
Tipo del documento:
Article