Effect of parathyroid hormone (1-34) on expression of matrix Gla protein and Wnt/β catenin signaling pathways in MG63 cell lines / 南方医科大学学报
Journal of Southern Medical University
; (12): 984-989, 2016.
Article
en Zh
| WPRIM
| ID: wpr-286861
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of parathyroid hormone (PTH)(1-34) on the expression of matrix Gla protein (MGP) and Wnt/β-catenin signaling pathway and elucidate the possible molecular mechanism of PTH (1-34) in the prevention and treatment of osteoporosis.</p><p><b>METHODS</b>MG63 cells treated with PTH (1-34) at 10(-9), 10(-8), and 10(-7) mol/L, alone or in combination with Wnt/β-catenin signaling pathway inhibitors DKK-1 (200 ng/ml) were examined for mRNA and protein expressions related with Wnt/β-catenin signaling with real-time PCR and Western blotting. The cell differentiation after the treatment was assessed with alkaline phosphatase (ALP) staining and cell viability assay.</p><p><b>RESULTS</b>PTH (1-34) significantly increased the expression of MGP in a dose-dependent manner in MG63 cells (P<0.05 or P<0.01). PTH treatment obviously enhanced ALP activity in the cells, and this effect was suppressed by DKK-1. Combined treatment with DKK-1 partially blocked PTH-induced enhancement of ALP activity (P<0.05). PTH promoted the expression of MGP and enhanced LRP5, β-catenin, and Runx2 expressions in Wnt/β-catenin signaling pathway at both protein and mRNA levels (P<0.05 or P<0.01). DKK-1 partially blocked the effect of PTH (1-34) on Wnt/β-catenin signaling pathway (P<0.05) without affecting MGP expression.</p><p><b>CONCLUSION</b>PTH (1-34) significantly increases the expressions of MGP and proteins in the Wnt/β-catenin signaling pathway. Wnt/β-catenin signaling pathway and MGP mediate the regulation of osteogenosis by PTH.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Osteogénesis
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Osteoporosis
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Hormona Paratiroidea
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Farmacología
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Proteínas de Unión al Calcio
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Diferenciación Celular
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Supervivencia Celular
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Proteínas de la Matriz Extracelular
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Péptidos y Proteínas de Señalización Intercelular
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Línea Celular Tumoral
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Southern Medical University
Año:
2016
Tipo del documento:
Article