Sequencing and analysis of the complete genome sequence of WU polyomavirus in Fuzhou, China / 病毒学报
Chinese Journal of Virology
; (6): 165-169, 2011.
Article
en Zh
| WPRIM
| ID: wpr-286060
Biblioteca responsable:
WPRO
ABSTRACT
WU polyomavirus (WUPyV), a new member of the genus Polyomavirus in the family Polyomaviridae, is recently found in patients with respiratory tract infections. In our study, the complete genome of the two WUPyV isolates (FZ18, FZTF) were sequenced and deposited in GenBank (accession nos. FJ890981, FJ890982). The two sequences of the WUPyV isolates in this study varied little from each other. Compared with other complete genome sequences of WUPyV in GenBank (strain B0, S1-S4, CLFF, accession nos. EF444549, EF444550, EF444551, EF444552, EF444553, EU296475 respectively), the sequence length in nucleotides is 5228bp, 1bp shorter than the known sequences. The deleted base pair was at nucleotide position 4536 in the non-coding region of large T antigen (LTAg). The genome of the WUPyV encoded for five proteins. They were three capsid proteins: VP2, VP1, VP3 and LTAg, small T antigen (STAg), respectively. To investigate whether these nucleotide sequences had any unique features, we compared the genome sequence of the 2 WUPyV isolates in Fuzhou, China to those documented in the GenBank database by using PHYLIP software version 3.65 and the neighbor-joining method. The 2 WUPyV strains in our study were clustered together. Strain FZTF was more closed to the reference strain B0 of Australian than strain FZ18.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Filogenia
/
Datos de Secuencia Molecular
/
China
/
Genoma Viral
/
Análisis de Secuencia de ADN
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Evolución Molecular
/
Genómica
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Polyomaviridae
/
Genética
/
Métodos
Límite:
Adult
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Child, preschool
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Humans
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Male
País/Región como asunto:
Asia
Idioma:
Zh
Revista:
Chinese Journal of Virology
Año:
2011
Tipo del documento:
Article