Heme oxygenase-1 expression and apoptosis induced by cadmium in human embryon kidney cells / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
; (12): 16-19, 2006.
Article
en Zh
| WPRIM
| ID: wpr-285818
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate apoptosis and expression of heme oxygenase-1 (HO-1) induced by cadmium in human embryonic kidney cells (HEK239T).</p><p><b>METHODS</b>The MTT method was used for determining the cell proliferation activity. The apoptosis was determined by the flow cytometry. The HO-l mRNA expression and protein level were detected by RT-PCR method and Western blot respectively.</p><p><b>RESULTS</b>The ratios of apoptosis in HEK239T cells were 11.90% +/- 0.28%, 9.27% +/- 1.73%, 9.79% +/- 0.67% and 8 .97% +/- 1.60% at the concentration of 5.0, 10.0, 20.0 and 40.0 micromol/L CdCl(2) respectively, higher than those in the control group (6.69% +/- 0.46%) with the significant difference (P < 0.01). The CdCl(2) of between 10 and 40 micromol/L could highly induce the expression of HO-1 of the human embryonic kidney cells. The expression would increase slowly till the flat stage with the increase of the dosage and then would decrease slightly over time.</p><p><b>CONCLUSION</b>The cadmium can induce the apoptosis of the human embryonic kidney cells and up-regulate the expression of HO-1.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Farmacología
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Cadmio
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ARN Mensajero
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Células Cultivadas
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Embriología
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Apoptosis
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Biología Celular
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Relación Dosis-Respuesta a Droga
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Hemo-Oxigenasa 1
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Genética
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Año:
2006
Tipo del documento:
Article