Protein kinase C enhances the swelling-induced chloride current in human atrial myocytes / 华中科技大学学报（医学）（英德文版）
J. huazhong univ. sci. tech. med. sci
; (6): 383-388, 2016.
Article
en En
| WPRIM
| ID: wpr-285258
Biblioteca responsable:
WPRO
ABSTRACT
Swelling-activated chloride currents (ICl.swell) are thought to play a role in several physiologic and pathophysiologic processes and thus represent a target for therapeutic approaches. However, the mechanism of ICl.swell regulation remains unclear. In this study, we used the whole-cell patch-clamp technique to examine the role of protein kinase C (PKC) in the regulation of ICl.swell in human atrial myocytes. Atrial myocytes were isolated from the right atrial appendages of patients undergoing coronary artery bypass and enzymatically dissociated. ICl.swell was evoked in hypotonic solution and recorded using the whole-cell patch-clamp technique. The PKC agonist phorbol dibutyrate (PDBu) enhanced ICl.swell in a concentration-dependent manner, which was reversed in isotonic solution and by a chloride current inhibitor, 9-anthracenecarboxylicacid. Furthermore, the PKC inhibitor bis-indolylmaleimide attenuated the effect and 4α-PDBu, an inactive PDBu analog, had no effect on ICl.swell. These results, obtained using the whole-cell patch-clamp technique, demonstrate the ability of PKC to activate ICl,swell in human atrial myocytes. This observation was consistent with a previous study using a single-channel patch-clamp technique, but differed from some findings in other species.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Farmacología
/
Fisiología
/
Proteína Quinasa C
/
Forbol 12,13-Dibutirato
/
Cloruros
/
Transporte Iónico
/
Canales de Cloruro
/
Técnicas de Placa-Clamp
/
Medios de Cultivo
/
Biología Celular
Límite:
Humans
Idioma:
En
Revista:
J. huazhong univ. sci. tech. med. sci
Año:
2016
Tipo del documento:
Article