Effect of puerarin on PI3K/AKT pathway-mediated apoptosis of PASMCs / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 3041-3046, 2015.
Article
en Zh
| WPRIM
| ID: wpr-284802
Biblioteca responsable:
WPRO
ABSTRACT
To discuss the effect of puerarin (Pue) on the proliferation of hypoxia-induced pulmonary artery smooth muscle cells (PASMCs) and discuss whether the extracellular signal PI3K/AKT pathway was involved in the Pue-induced PASMC apoptosis. With the serum starvation group (SD group) as the control group, the MTT colorimetry method, Annexin V-FITC apoptosis detection kit and Western blot were used to detect Pue's effect on apoptosis of rat PASMCs. The protein immunoblot assay was used to detect whether PI3K/AKT pathway was involved in the inhibition of hypoxia-induced PASMC apoptosis process. The results show that under normoxic conditions, Pue had no effect on PASMC apoptosis; Under hypoxia conditions, Pue can inhibit PASMC apoptosis; Under normoxic and hypoxic conditions, Pue had no effect on TNF-α expression. Pue can reverse hypoxia-induced Bcl-2 (P <0.01), up-regulate it and down-regulated Bax (P <0.01). Under normoxic conditions, Pue had no effect on P-AKT expression. Both LY294002 and Pue can inhibit hypoxia-induced Bcl-2, up-regulation of P-AKT expression and down-regulation of Bax expression. Compared with the hypoxia + Pue group or the hypoxia + LY294002 group, the hypoxia + Pue + LY294002 group showed more significantly changes in Bcl-2, Bax, P-AKT expressions. The results show that, Pue can inhibit the hypoxic-induced PASMC apoptosis, which may be regulated through PI3K/AKT pathway.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Farmacología
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Fisiología
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Arteria Pulmonar
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Transducción de Señal
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Células Cultivadas
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Morfolinas
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Cromonas
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Ratas Wistar
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Apoptosis
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Fosfatidilinositol 3-Quinasas
Límite:
Animals
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Año:
2015
Tipo del documento:
Article