Isolation and identification of SARS virus in Guangdong province / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 213-216, 2003.
Article
en Zh
| WPRIM
| ID: wpr-281775
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>To isolate and identify pathogen of atypical pneumonia in Guangdong.</p><p><b>METHODS</b>Pathogens were isolated from variety of samples collected from atypical pneumonia patient by using MDCK cells, and identified with serological and molecular methods.</p><p><b>RESULTS</b>A novel coronavirus was isolated from patients with atypical pneumonia, from which an RNA fragment of 279 nt was amplified by nested RT-PCR. And sequence assay showed that only 39-65 percent of sequence of the virus was homogenous to known coronavirus, but almost 100% homogenous (with one base exception, 12a to t) to SARS-associated coronavirus isolated from patients outside Guangdong, such as in Beijing, Hong Kong, Taiwan, Germany, Italy and so on. Indirect immunofluorescence test showed a specific antigen-antibody reactivity between the coronavirus and convalescent-phase sera of SARS patients.</p><p><b>CONCLUSION</b>The pathogen of the atypical pneumonia in Guangdong province was a novel type of coronavirus, which could be isolated by using MDCK cells.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Filogenia
/
Neumonía Viral
/
Virología
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Línea Celular
/
China
/
Clasificación
/
Síndrome Respiratorio Agudo Grave
/
Coronavirus Relacionado al Síndrome Respiratorio Agudo Severo
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
/
Humans
País/Región como asunto:
Asia
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2003
Tipo del documento:
Article