Protective effect of ketamine against acute rat lung injury induced by liopolysaccharide and its mechanism / 南方医科大学学报
Journal of Southern Medical University
; (12): 1848-1850, 2007.
Article
en Zh
| WPRIM
| ID: wpr-281524
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the protective effects of different concentrations of ketamine against acute lung injury induced by lipopolysaccharide (LPS) in rats and its mechanism.</p><p><b>METHODS</b>Forty-eight male Wistar rats were randomized into 4 equal groups, namely the control group, LPS group, ketamine group I (5 mg/kg), and ketamine group II (10 mg/kg). The neutrophil count, protein contents in the bronchoalveolar lavage fluid (BALF) and the wet/dry lung weight ratio were measured 4 h after LPS injection. TNF-alpha, IL-8, NO, iNOS and NF-kappaB were also measured in the lung tissues.</p><p><b>RESULTS</b>In LPS group, the neutrophil count, protein contents in BALF, the wet/dry lung weight ratio and the levels of tumor necrosis factor-alpha(TNF-alpha), interleukin-8 (IL-8), and NO were all significantly increased compared with the control group (P<0.01). The mRNA expression of iNOS and the protein expression of NF-kappaB were also increased in LPS groups. Ketamine treatment attenuated the increase in wet/dry lung weight ratio, neutrophil count, and protein contents in BALF in a dose-dependent manner. Ketamine also dose-dependently inhibited the production of TNF-alpha, IL-8 , and NO and lowered iNOS mRNA and NF-kappaB protein expression.</p><p><b>CONCLUSION</b>Ketamine can offer protection against LPS-induced acute lung injury in rats by inhibiting the expression of NF-kappaB and attenuating the production of the inflammatory cytokines.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Patología
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Farmacología
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Líquido del Lavado Bronquioalveolar
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Lipopolisacáridos
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Interleucina-8
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FN-kappa B
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Factor de Necrosis Tumoral alfa
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Ratas Wistar
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Quimioterapia
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Óxido Nítrico Sintasa de Tipo II
Límite:
Animals
Idioma:
Zh
Revista:
Journal of Southern Medical University
Año:
2007
Tipo del documento:
Article