The role of CXCR1/2 in shear stress-induced endothelial cell migration / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 512-517, 2009.
Article
en Zh
| WPRIM
| ID: wpr-280164
Biblioteca responsable:
WPRO
ABSTRACT
CXCR1 and CXCR2 are important receptors in regulating vascular endothelial cell activities. In order to elucidate the role of CXCR1/2 in shear stress-induced endothelial cell migration, we have investigated the expression levels of CXCR1 and CXCR2 in the endothelial cells exposed to shear stress. In the experiment, anti-IL8RA and anti-IL8RB were used to antagonize CXCR1 and CXCR2. Different shear stresses were generated in a flow chamber; scratch test was carried out to compare endothelial cell migration in the control group and the receptor-antagonized groups. The results indicated that the migration of endothelial cells was restrained effectively after CXCR1 and CXCR2 were antagonized by anti-IL8RA and anti-IL8RB. And anti-IL8RA showed a stronger inhibitive effect than did anti-IL8RB (P<0.05). In the group with both receptor antagonisms, the migration was further inhibited. These results suggest that both CXCR1 and CXCR2 are important factors in mediating the migration of endothelial cells induced by shear stress, and CXCR1 fulfills a more important role.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Fisiología
/
Estrés Mecánico
/
Venas Umbilicales
/
Movimiento Celular
/
Receptores de Interleucina-8A
/
Receptores de Interleucina-8B
/
Biología Celular
/
Mecanotransducción Celular
/
Resistencia al Corte
/
Células Endoteliales
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Biomedical Engineering
Año:
2009
Tipo del documento:
Article