Mutations of HBV polymerase gene sequence in lamivudine-resistant chronic hepatitis B patients / 南方医科大学学报
Journal of Southern Medical University
; (12): 729-730, 2008.
Article
en Zh
| WPRIM
| ID: wpr-280109
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the characteristics of mutation in HBV polymerase (P) gene reverse transcriptase region (RT region) in lamivudine-resistant chronic hepatitis B (CHB) patients.</p><p><b>METHODS</b>This study involved 115 CHB patients who developed clinical resistance to lamivudine. Direct sequencing of the PCR products was used to detect lamivudine genotypic resistance.</p><p><b>RESULTS</b>Lamivudine resistant mutation was detected in 103 patients, and the major mutations included rtL180M+rtM204V and rtM204I, accounting for 58.3% and 22.3%, respectively. Other resistant substitutions included rtL80V/I, rtT184S, and rtA200V, and combined mutation of triple resistant substitutions was detected in HBV RT region of 5 patients by direct sequencing.</p><p><b>CONCLUSION</b>For lamivudine-treated patients, combined mutation at the sites other than rtL180 and rtM204 in HBV P gene should also be detected for drug resistance evaluation.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Virología
/
Productos del Gen pol
/
Virus de la Hepatitis B
/
Lamivudine
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Fármacos Anti-VIH
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Hepatitis B Crónica
/
Farmacorresistencia Viral
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Usos Terapéuticos
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Quimioterapia
/
Genética
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Southern Medical University
Año:
2008
Tipo del documento:
Article