Efficient expression of soluble human FGF-21 and its glucose regulation activity / 药学学报
Acta Pharmaceutica Sinica
; (12): 548-552, 2009.
Article
en Zh
| WPRIM
| ID: wpr-278223
Biblioteca responsable:
WPRO
ABSTRACT
The cDNA of human FGF-21 was subcloned into the pSUMO expression vector and the fusion protein was induced to express in Escherichia coli Rosetta (DE3). The recombinant hFGF-21 was expressed in soluble form in the pSUMO expression system. The recombinant fusion protein was purified by Ni-NTA column. The purified recombinant protein was dialyzed against PBS for re-nature. To obtain pure and active recombinant protein, the fusion protein was subjected to cleavage with SUMO protease I. To examine glucose regulation activity of hFGF-21, 3T3-L1 pre-adipocytes were differentiated into adipocytes, glucose up-take activity of hFGF-21 was examined by glucose oxidase and peroxidase (GOD-POD) assay. Compared with no stimulation control, the recombinant hFGF-21 treatment led to a significant increase in glucose consumption of adipocytes and a significant decrease in concentration of glucose in the medium (P < 0.05, P < 0.001).
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Farmacología
/
Plásmidos
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Solubilidad
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Proteínas Recombinantes de Fusión
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ADN Complementario
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Adipocitos
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Proteínas Modificadoras Pequeñas Relacionadas con Ubiquitina
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Células 3T3-L1
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Escherichia coli
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Factores de Crecimiento de Fibroblastos
Límite:
Animals
/
Humans
Idioma:
Zh
Revista:
Acta Pharmaceutica Sinica
Año:
2009
Tipo del documento:
Article