Identification of oligopeptides binding to Mycoplasma hyorhinis P37 using a phage display library / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 1165-1188, 2011.
Article
en Zh
| WPRIM
| ID: wpr-274933
Biblioteca responsable:
WPRO
ABSTRACT
Phage display random heptapeptide library was screened with recombinant P37 in this study. The positive phage clones were identified by ELISA and were sequenced, and the amino acid sequences of the polypeptides displayed on phage were deduced. After GST-polypeptides fusion protein was constructed and expressed, its binding to P37 was determined by GST-pull down and Western blot. After 4 rounds of bio-panning, the enriched positive phage clones were identified by ELISA. Eighteen positive phage clones were sequenced and the peptide sequences were as follows. ACAPKPPWLC (12/18), RPLSIDPWSPHL (3/18), RPLSNDPWSPHL (1/18), QNMMSPIEGVRI (1/ 18) and WAPEKDYMQLMK (1/18). The results from GST-pull down and Western blot showed that peptide RPLSIDPWSPHL could interact with P37. The study will be helpful for identifying the protein reacting with P37.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Oligopéptidos
/
Unión Proteica
/
Porcinos
/
Proteínas Bacterianas
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Biblioteca de Péptidos
/
Mycoplasma hyorhinis
/
Metabolismo
Límite:
Animals
Idioma:
Zh
Revista:
Journal of Biomedical Engineering
Año:
2011
Tipo del documento:
Article