Roles of SPK in Regulation of Hypoxia Induced Proliferation and Glucose Consume of Leukemia Cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 965-969, 2017.
Article
en Zh
| WPRIM
| ID: wpr-271885
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To clarify the roles of SPK pathway in the regulation of proliferation, survival and glucose consume of human erythroleukemia TF-1 cells.</p><p><b>METHODS</b>The interfering in SPK expression of TF-1 cells was performed using leutivirus vector-mediated shRNA, the interference efficacy of SPK in TF-1 cells was detected by RT-qPCR and Western blot, the viability of TF-1 cell proliferation was detected by using CCK-8 method, the apoptosis of TF-1 cells was determined by flow cytmetry with Annexin V staining.</p><p><b>RESULTS</b>Hypoxia up-regulated the expression of HIF-1α, HIF-2α, and SPK in TF-1 cells. SPK treatment resulted in reduced proliferation and induced apoptosis in TF-1 cells. Furthermore, knockdown of the SPK significantly reduced utilization and consumption of glucose.</p><p><b>CONCLUSION</b>The SPK is key signalling molecule involved in regulation of hypoxia-induced proliferation and glucose metabolism in TF-1 cells, and plays an important rote in proliferation and energy metabolism of leukemia cells.</p>
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Base de datos:
WPRIM
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Año:
2017
Tipo del documento:
Article