Effect of malathion on the male reproductive organs of earthworms, Eisenia foetida / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
; (6): 97-101, 2005.
Article
en En
| WPRIM
| ID: wpr-270866
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>AIM</b>To observe the cytotoxic effect of the organophosphate insecticide malathion in the reproductive tissues of the earthworms, Eisenia foetida.</p><p><b>METHODS</b>Worms were nourished in soil treated with malathion at single sub-lethal doses of 0, 80, 150, 300 and 600 mg/kg soil. (LD50=880 mg/kg soil) and evaluated on days 1, 5, 15 and 30 after exposure. The body weights were recorded and male reproductive organs evaluated.</p><p><b>RESULTS</b>Malathion-treated animals showed a significant reduction in body weight in a dose-dependent manner. Malathion treatment modified the disposition of spermatozoa in the basal epithelium of the spermatheca. The Br-deoxyuridine test showed a significant rise in cells in phase S on days 5 and 15. Also, a higher percentage of spermatogonia with fragmented DNA were observed by means of the TdT-mediated dUTP nick-end labeling (TUNEL) technique in the spermatheca of treated animals.</p><p><b>CONCLUSION</b>Treatment with malathion decreased the body weight and the spermatic viability in spermatheca, altering the cell proliferation and modifying the DNA structure of spermatogonia.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Oligoquetos
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Reproducción
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Espermatozoides
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Factores de Tiempo
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Peso Corporal
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Fase S
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Etiquetado Corte-Fin in Situ
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Relación Dosis-Respuesta a Droga
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Fragmentación del ADN
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Genética
Límite:
Animals
Idioma:
En
Revista:
Asian Journal of Andrology
Año:
2005
Tipo del documento:
Article