Estradiol stimulates proliferation of prostatic smooth muscle cells via estrogen receptor alpha and IGF1 / 中华男科学杂志
National Journal of Andrology
; (12): 131-135, 2011.
Article
en Zh
| WPRIM
| ID: wpr-266200
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of estrogen receptor alpha (ERa) and insulin-like growth factor 1 (IGF1) on the proliferation of prostatic smooth muscle cells (PSMCs) in vitro.</p><p><b>METHODS</b>The ERalpha shRNA expression frame was subcloned to the pGSadeno adenovirus vector by homologous recombination technology to construct the pGSaaeno-ERalpha vector. After the mouse PSMCs were transfected in vitro by pGSaaeno-ERalpha, the mRNA and protein expression levels of ERalpha were detected by RT-PCR and Western blot respectively. The expression of IGF1 in the ERa-reduced cells was determined by Western blot 6 hours after treatment with 17beta-estradiol (E2) at 10(-8) mol/L. The post-transfection activity of estrogen or exogenous IGF1 in the proliferation of PSMCs was evaluated by MTT chlormetric analysis.</p><p><b>RESULTS</b>After treatment with E2, the proliferation of PSMCs and the expression of the IGF1 gene were significantly increased in the normal control group (P <0.05), but not obviously changed in the ERalpha-siRNA group (P> 0.05). And exogenous IGF1 failed to induce the proliferation of the ERalpha-reduced PSMCs.</p><p><b>CONCLUSION</b>E2 induces the expression of IGF1 via ERalpha, and IGFl, with the interaction of ERalpha, promotes the proliferation of PSMCs.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Farmacología
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Próstata
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ARN Mensajero
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Factor I del Crecimiento Similar a la Insulina
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Células Cultivadas
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Biología Celular
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Miocitos del Músculo Liso
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Receptor alfa de Estrógeno
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Proliferación Celular
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Estradiol
Límite:
Animals
Idioma:
Zh
Revista:
National Journal of Andrology
Año:
2011
Tipo del documento:
Article