A PCR-based Rapid Neutralization Assay for GII.4 Norovirus Infection in HIEC6 Cell Culture / 生物医学与环境科学（英文）
Biomedical and Environmental Sciences
; (12): 219-221, 2015.
Article
en En
| WPRIM
| ID: wpr-264597
Biblioteca responsable:
WPRO
ABSTRACT
Because of limited viral replication and lack of cytopathic effect in cell culture, a new PCR-based rapid seroneutralization assay for detection of GII.4 norovirus neutralized antibodies was developed with serum samples from acute-phase patients, convalescent-phase patients and healthy controls. According to this study, neutralizing antibodies were detected in 100% of convalescent-phase sera, and in 2.5% of healthy controls sera. However, all of the acute-phase serum samples could not neutralize virus efficiently. Compared to the results from ELISA (96.2% at sensitivity and 80% at specificity), the present in vitro neutralization assay is more specific and more sensitive.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Virología
/
Ensayo de Inmunoadsorción Enzimática
/
Secuencia de Bases
/
Estudios de Casos y Controles
/
Línea Celular
/
Reacción en Cadena de la Polimerasa
/
Sensibilidad y Especificidad
/
Cartilla de ADN
/
Infecciones por Caliciviridae
/
Norovirus
Tipo de estudio:
Diagnostic_studies
/
Observational_studies
Límite:
Humans
Idioma:
En
Revista:
Biomedical and Environmental Sciences
Año:
2015
Tipo del documento:
Article