Expression of CD14 and Toll-like receptor 4 on Kupffer cells and its role in ischemia-reperfusion injury on rat liver graft / 中华外科杂志
Chinese Journal of Surgery
; (12): 274-276, 2005.
Article
en Zh
| WPRIM
| ID: wpr-264526
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression of lipopolysaccharide (LPS) receptor CD14 and Toll-like receptor 4 (TLR4) on Kupffer cells and its role in ischemia-reperfusion injury (IRI) on rat liver graft.</p><p><b>METHODS</b>The Kupffer cells following IRI were isolated and divided into control, ischemia-reperfusion (IR), and anti-CD14 antibody group. The mRNA and protein expression of CD14 and TLR4, nuclear factor kappa B (NF-kappaB) activity and TNF-alpha level in supernatant were measured.</p><p><b>RESULTS</b>The mRNA and protein expression of CD14 and TLR4 in IR group were significantly higher than those in control group (P < 0.01). The NF-kappaB activity and TNF-alpha level in IR group were significantly higher than those in control group (P < 0.01), and they greatly decreased after anti CD14 antibody treatment (compared with IR group, P < 0.05), but were still significantly higher than those in control group (P < 0.01).</p><p><b>CONCLUSIONS</b>LPS following IRI could up-regulate CD14 and TLR4 gene and protein expression on Kupffer cells, and subsequently activate NF-kappaB to produce cytokines, but other signal transduction pathways might also participate in the IRI.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Patología
/
Fisiología
/
Técnicas In Vitro
/
ARN Mensajero
/
Daño por Reperfusión
/
Transducción de Señal
/
FN-kappa B
/
Trasplante de Hígado
/
Factor de Necrosis Tumoral alfa
/
Ratas Wistar
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Surgery
Año:
2005
Tipo del documento:
Article