Anti-inflammatory effect and mechanism of artemisinin and dihydroartemisinin / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2618-2621, 2012.
Article
en Zh
| WPRIM
| ID: wpr-263875
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study and compare the anti-inflammatory effect and molecular mechanism of artemisinin and dihydroartemisinin.</p><p><b>METHOD</b>Mouse mononuclear macrophage RAW264.7 cells were stimulated to release inflammatory mediators such as TNF-alpha, IL-6 and NO, in order to assess the drugs' inhibitory effect on macrophage's release of above inflammatory mediators. The levels of TNF-alpha and IL-6 were determined by ELISA and the cytotoxicity was determined by MTT method. The protein expression of iNOS, COX-2 and beta-actin were tested by Western blot. The enzymatic activity of COX-2 was determined by colorimetric method.</p><p><b>RESULT</b>Dihydroartemisinin significantly inhibited LPS-induced release of TNF-alpha, IL-6 and NO from RAW264.7 in mice with the concentration range of 12.5 - 100 micromol x L(-1), and showed good dose dependence. Artemisinin only inhibited the IL-6 release to a certain extent.</p><p><b>CONCLUSION</b>Dihydroartemisinin inhibits macrophages from releasing inflammatory factors TNF-alpha and IL-6 and inflammatory mediators NO by down-regulating iNOS protein. Artemisinin may help dihydroartemisinin to show its anti-inflammatory effect through metabolism.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Farmacología
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Expresión Génica
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Línea Celular
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Interleucina-6
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Factor de Necrosis Tumoral alfa
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Mediadores de Inflamación
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Artemisininas
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Alergia e Inmunología
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Genética
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Macrófagos
Límite:
Animals
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Año:
2012
Tipo del documento:
Article