Comparison of multilocus sequence typing system and pulsed-field gel electrophoresis in typing of Salmonella enteritidis / 中华流行病学杂志
Chinese Journal of Epidemiology
; (12): 1065-1068, 2006.
Article
en Zh
| WPRIM
| ID: wpr-261674
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To compare the discriminatory ability between multilocus sequence typing system (MLST) and pulsed-field gel electrophoresis (PFGE).</p><p><b>METHODS</b>Salmonella enteritidis strains, isolated from food in China were identified by MLST, under PCR products of thrA, purE, sucA, aroC, hemD, dnaN and Sdf I. The same set of strains was typed by PFGE using Xba I, Spe I as the restriction enzyme in order to compare the discriminatory power of the methods.</p><p><b>RESULTS</b>Data from MLST revealed the lack of diversity among the strains of the same serotype and the number of variable nucleotide sites per locus ranged from 1 to 23 between Salmonella typhi LT2 and other serotypes of Salmonella. However, 50 Salmonella enteritidis strains were identified as 11 patterns and more sub-patterns by PFGE.</p><p><b>CONCLUSION</b>In strain typing, PFGE was the highly discriminatory method comparing to the MLST system.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Salmonella enteritidis
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Serotipificación
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Reacción en Cadena de la Polimerasa
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Clasificación
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Electroforesis en Gel de Campo Pulsado
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Análisis de Secuencia de ADN
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Genes Bacterianos
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Genética
Tipo de estudio:
Prognostic_studies
Idioma:
Zh
Revista:
Chinese Journal of Epidemiology
Año:
2006
Tipo del documento:
Article