Construction and expression of repeats of HER-2 mimetic peptide / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 1076-1099, 2010.
Article
en Zh
| WPRIM
| ID: wpr-260935
Biblioteca responsable:
WPRO
ABSTRACT
To produce many epitopes of peptide mut, different tandem repeats containing HER-2 mimetic peptide mut were constructed and expressed. The oligonucleotide coding mut sequence was inserted into pET28a(+) to construct the prokaryotic recombinant plasmid pET28a-mutl. The mut coding sequence was repeatedly inserted into the vector in tandem to obtain a series of plasmids pET28a-mut2, pET28a-mut4, pET28a-mut8 and pET28a-mut16. The plasmids were transformed into E. coli. BL21 and induced by IPTG. The recombinant proteins were expressed in E. coli. The binding analysis verified that the interaction between the tandem peptides mut and Herceptin was increased. In conclusion, the tandem peptides consisted of many antigen epitopes, and laid the foundation for the further study of HER-2 peptide vaccine for biotherapy of cancer with HER-2 overexpression.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Péptidos
/
Proteínas Recombinantes
/
Neoplasias de la Mama
/
Expresión Génica
/
Química
/
Receptor ErbB-2
/
Vacunas contra el Cáncer
/
Alergia e Inmunología
/
Escherichia coli
/
Genética
Límite:
Female
/
Humans
Idioma:
Zh
Revista:
Journal of Biomedical Engineering
Año:
2010
Tipo del documento:
Article