Construction of eukaryotic expression vector of LYRM1, a novel gene related with human obesity, and establishment of its stable transfected 3T3-L1 cell line / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 493-497, 2009.
Article
en Zh
| WPRIM
| ID: wpr-259277
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To construct the eukaryotic expression vector of LYRM1 and to transfect it to preadipocytes cell line 3T3-L1.</p><p><b>METHODS</b>The complete coding sequence of LYRM1 gene was amplified by RT-PCR from human omental adipose tissue and was subcloned into eukaryotic expression vector pcDNA(TM)3.1/myc-His B. The recombinant plasmid was then transfected into 3T3-L1 preadipocytes by liposome. After screening culture by G418, stable transfected 3T3-L1 cell line was established. The expression of LYRM1 protein was identified by Western blot.</p><p><b>RESULT</b>The recombinant plasmid was confirmed by PCR, restriction enzyme digestion and sequencing. The stable transfected 3T3-L1 cell line was established and the LYRM1 protein was expressed successfully.</p><p><b>CONCLUSION</b>The eukaryotic expression vector of LYRM1 has been successfully established and the stably transfected 3T3-L1 cell line also established.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Proteínas Recombinantes
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Transfección
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Tejido Adiposo
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Células 3T3-L1
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Proteínas Reguladoras de la Apoptosis
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Vectores Genéticos
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Genética
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Metabolismo
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Obesidad
Tipo de estudio:
Prognostic_studies
Límite:
Animals
/
Humans
Idioma:
Zh
Revista:
Journal of Zhejiang University. Medical sciences
Año:
2009
Tipo del documento:
Article