Effect of PC cell-derived growth factor RNA interference on biological behavior of esophageal squamous carcinoma cells / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
; (12): 874-877, 2013.
Article
en Zh
| WPRIM
| ID: wpr-256900
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of PC cell-derived growth factor (PCDGF) RNA interference on esophageal squamous carcinoma cells Eca-109 in vitro.</p><p><b>METHODS</b>The PCDGF-shRNA expression vector was transfected into the Eca-109 cells by liposome. After transfection, the mRNA and protein expressions of PCDGF were detected by RT-PCR and Western-blot respectively. Cell Counting Kit-8 (CCK-8) assay and Boyden chamber method were performed to measure the cell proliferation and invasion ability respectively.</p><p><b>RESULTS</b>The expression levels of PCDGF mRNA and protein were both decreased in Eca-109 cells transfected with PCDGF-shRNA expression vector (transfection group). Twenty-four, 48 and 72 h after transfection, the cells proliferation in the transfection group was inhibited, and the inhibition rate was 20.4%, 21.1% and 20.9% respectively. The cell proliferation activity in the transfection group was significantly lower than that in the non-transfection group, liposome group and negative vector group (all P<0.05). The number of cell migration in the non-transfection group,negative vector group, liposome group and transfection group was 118.8±12.0, 100.8±9.0, 114.3±4.7, and 53.5±16.3 respectively. The differences were statistically significant between the transfection group and the other 3 groups (all P<0.05).</p><p><b>CONCLUSIONS</b>PCDGF RNA interference can inhibit the proliferation and invasion abilities of esophageal squamous carcinoma cells in vitro. PCDGF gene may be the new target of gene therapy.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Patología
/
Neoplasias Esofágicas
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Carcinoma de Células Escamosas
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Transfección
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Péptidos y Proteínas de Señalización Intercelular
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ARN Interferente Pequeño
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Interferencia de ARN
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Línea Celular Tumoral
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Proliferación Celular
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Vectores Genéticos
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Gastrointestinal Surgery
Año:
2013
Tipo del documento:
Article