Study on purification of total flavonoids from seed residue of Hippophae rhamnoides with macroporous resin / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 225-228, 2004.
Article
en Zh
| WPRIM
| ID: wpr-256386
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the conditions and parameters of purifying total flavonoids from seed residue of H. rhamnoides.</p><p><b>METHOD</b>Absorption capacity of three resins for total flavonoids was compared. With the yield and purity as indexes, the process of absorbing and purifying total flavonoids from seed residue with D101 macroporous resin absorbent was selected by orthogonal design.</p><p><b>RESULT</b>The D101 resin was the best of the three resins and its absorption capacity was determined to be 63.00 mg x g(-1) wet resin. The optimum process condition was 30% ethanol as eluting solvent, wet resin vs crude herbs: 2:1, diameter vs height: 1:10, eluting solvent vs crude herbs: 10:1, absorbing time for 3 h. The yield of total flavonoids from seeds residue of H. rhamnoides by this process was 2.39%, and the purity reached 64.81%.</p><p><b>CONCLUSION</b>The process is simple and convenient and the regeneration of resin is easy. So this method of purification is advisable.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Plantas Medicinales
/
Resinas Sintéticas
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Semillas
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Flavonoides
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Química
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Tecnología Farmacéutica
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Hippophae
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Etanol
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Métodos
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Año:
2004
Tipo del documento:
Article