Detection of mdr1 gene by real-time fluorescence quantitative polymerase chain reaction using Taq Man-MGB probe / 南方医科大学学报
Journal of Southern Medical University
; (12): 466-468, 2006.
Article
en Zh
| WPRIM
| ID: wpr-255283
Biblioteca responsable:
WPRO
ABSTRACT
Primer Express 2.0 software was used to design the primers and the MGB probe. With the plasmid pHaMDR1/A containing mdr1 cDNA as the template, we established a real-time fluorescent quantitative polymerase chain reaction system, which, at the template concentration of 3.061 x 10(3) to 3.061 x 10(9) cps/ml, had a correlation coefficient of 0.988243 between template concentration and threshold cycle value. This PCR method allows sensitive, specific and quantitative detection of human mdr1 gene.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Reacción en Cadena de la Polimerasa
/
Cartilla de ADN
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Miembro 1 de la Subfamilia B de Casetes de Unión a ATP
/
Genes MDR
/
Polimerasa Taq
/
Colorantes Fluorescentes
/
Fluorometría
/
Genética
/
Métodos
Tipo de estudio:
Diagnostic_studies
Límite:
Female
/
Humans
/
Male
Idioma:
Zh
Revista:
Journal of Southern Medical University
Año:
2006
Tipo del documento:
Article