Expression of plateau adaptation gene of rat tissues after plain acute exposure to high altitude / 浙江大学学报·医学版
Zhejiang Daxue xuebao. Yixue ban
; (6): 571-577, 2015.
Article
en Zh
| WPRIM
| ID: wpr-255152
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To detect the expression of the plateau adaptablity gene(EPAS1, EGLN1 and PPARα) and proteins(HIF-2, PHD2 and PPARα) in rats blood, heart, liver, lung and kidney tissue after the rats exposed to high altitude.</p><p><b>METHODS</b>The Wistar rats were randomly divided into plain group(Shanghai, 55 m), acute exposure to high altitude 3400 m group, acute exposure to high altitude 4300 m group. Blood and organs of rats were collected in 1, 3, 5 days after arrival. Real time PCR and ELISA were used to compare the expression of plateau adaptablity gene and related protein between plain group and high altitude exposure groups.</p><p><b>RESULTS</b>The count of red blood cells, hemoglobin and HCT in high altitude 4300 m were higher than those in plain group. Compared with plain group, the expression of EPAS1 gene in blood, heart, liver and kidney tissue of rats at high altitude increased obviously(all P<0.05); the expression of EGLN1 in the heart, liver, brain and kidney increased, and PPARα gene in the heart, liver and kidney increased(all P<0.05). Compared with plain group, the expression of HIF-2 protein increased significantly at high altitudes in the liver, brain and kidney tissues. PHD2 and PPARα increased in the heart, liver and kidney.</p><p><b>CONCLUSION</b>Plateau adaptive genes(EPAS1, EGLN1 and PPARα) and protein(HIF-2, PHD2 and PPARα) differed in different altitude and different organizations. They might be used as target markers of plateau hypoxia.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Encéfalo
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Adaptación Fisiológica
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China
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Ratas Wistar
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Procolágeno-Prolina Dioxigenasa
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PPAR alfa
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Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico
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Altitud
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Prolina Dioxigenasas del Factor Inducible por Hipoxia
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Corazón
Límite:
Animals
País/Región como asunto:
Asia
Idioma:
Zh
Revista:
Zhejiang Daxue xuebao. Yixue ban
Año:
2015
Tipo del documento:
Article