Designation and evaluation of antisense oligodeoxynucleotides targeted to glial glutamate transporter-1a / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 238-243, 2015.
Article
en Zh
| WPRIM
| ID: wpr-255043
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>The present study was undertaken to design antisense oligodeoxynucleotides (AS-ODNs) of glial glutamate transporter-la (GLT-1a) and to evaluate the effectiveness of the designed AS-ODNs on the expression of GLT-1a.</p><p><b>METHODS</b>Five sequences of GLT-1a AS-ODNs were designed according to the C terminus specific sequences of GLT-1a mRNA using antisense design software of IDT Com- pany. Western blot analysis was used to evaluate the inhibition effects of the five GLT-1a AS-ODNs on the expression of GLT-la.</p><p><b>RESULTS</b>The sequence of GLT-1a AS-ODNs with sequence of 5'-GGTTCTTCCTCAACACTGCA-3' could specifically inhibit the expression of GLT-1a in the hippocampal CA1 subfield of rats, while it had no effect on the expression of GLT-1b. This sequence showed similar inhibition on the expression of GLT-la in sham and ceftriaxone (Cef)-treated rats. It could also significantly inhibit the cerebral ischemic preconditioning (CIP)-induced up-regulation in the expression of GLT-1a. The magnitude of the inhibition in sham, Cef- or CIP-treated rats was similar by more than 60%.</p><p><b>CONCLUSION</b>From the designed five sequences of GLT-1a AS-ODNs, we obtained an effective sequence which can specifically inhibit the expression of GLT-1a.</p>
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Base de datos:
WPRIM
Asunto principal:
ARN Mensajero
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Regulación hacia Arriba
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Oligonucleótidos Antisentido
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Precondicionamiento Isquémico
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Transportador 2 de Aminoácidos Excitadores
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Región CA1 Hipocampal
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Genética
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Metabolismo
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Applied Physiology
Año:
2015
Tipo del documento:
Article