Correlation of expression of RhoA (RhoC and their effector ROCK-1 with malignant phenotype of ovarian cancer cells in vitro / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 385-388, 2004.
Article
en Zh
| WPRIM
| ID: wpr-254328
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of RhoA, RhoC and their effector ROCK-1 in four ovarian cancer cell lines in vitro and their correlation with invasiveness.</p><p><b>METHODS</b>Expression of RhoA, RhoC and ROCK-1 mRNA and protein in four ovarian cancer cell lines SW626, Skov-3, A2780 and Caov-3 was detected by RT-PCR and Western blot assay. Invasion assay was done in Boyden chamber.</p><p><b>RESULTS</b>The expression levels of RhoA, RhoC and ROCK-1 mRNA and protein varied in the four different cell lines examined. The expression level of RhoC, but not RhoA and ROCK-1, was significantly correlated with the invasive capability of these cells in vitro (r = 0.95, P < 0.01). Expression of RhoA at the level of transcription was not correlated with that at the translation level. The expression of RhoA and RhoC did not correlate with that of ROCK-1.</p><p><b>CONCLUSION</b>Expression level of RhoC may serve as an independent parameter in evaluating metastasis and become a new target in inhibiting ovarian cancer metastasis.</p>
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Base de datos:
WPRIM
Asunto principal:
Neoplasias Ováricas
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Patología
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Fenotipo
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Transcripción Genética
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Biosíntesis de Proteínas
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ARN Mensajero
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Regulación Neoplásica de la Expresión Génica
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Movimiento Celular
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Proteínas Serina-Treonina Quinasas
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Proteínas de Unión al GTP rho
Límite:
Female
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Humans
Idioma:
Zh
Revista:
Chinese Journal of Oncology
Año:
2004
Tipo del documento:
Article