Effect of hyperoxia on p53 and PCNA expression in fetal rat lung fibroblasts / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
; (12): 922-926, 2014.
Article
en Zh
| WPRIM
| ID: wpr-254167
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of hyperoxia exposure on the expression of p53 and proliferating cell nuclear antigen (PCNA) in fetal rat lung fibroblasts (LFs).</p><p><b>METHODS</b>Primary rat embryonic LFs were cultured in vitro. LFs grew to subconfluence and then were randomly divided into air and hyperoxia exposure (95% O₂, 5% CO₂) groups. After LFs were cultured for 12 and 24 hours, the proliferation was analyzed by MTT. p53 mRNA level was detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). p53 and PCNA protein levels were determined by Western blot.</p><p><b>RESULTS</b>After 12 and 24 hours of culture the growth inhibition rate of LFs was 8% and 23% respectively in the hyperoxia exposure group. p53 mRNA and protein levels increased significantly (P<0.01) in the hyperoxia exposure group after 12 and 24 hours of culture compared with the air exposure group. Hyperoxia exposure decreased PCNA expression after 24 hours of culture (P<0.01).</p><p><b>CONCLUSIONS</b>Hyperoxia exposure increases p53 level and decreases PCNA expression, resulting in inhibitions of LFs proliferation and DNA repair.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Patología
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ARN Mensajero
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Proteína p53 Supresora de Tumor
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Ratas Sprague-Dawley
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Antígeno Nuclear de Célula en Proliferación
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Hiperoxia
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Biología Celular
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Proliferación Celular
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Fibroblastos
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Genética
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Contemporary Pediatrics
Año:
2014
Tipo del documento:
Article