Antigenic properties of mutant hepatitis B virus surface antigen / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 48-50, 2008.
Article
en Zh
| WPRIM
| ID: wpr-254144
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the antigenic properties of mutant hepatitis B virus surface antigen, to understand the sensitivity of the commercially available HBsAg assays to the variants and to reduce the undetectability of the variants.</p><p><b>METHODS</b>Recombinant eukaryotic expression plasmids for HBsAg. The recombinant eukaryotic expression plasmids pSS1adr, pSS1adw2, pSS1adw2- 145Arg, pSS1adr-126 Asn and pSS1adr-126Ser were transfected into COS-7 cells. HBsAg in the supernatants of transfected cells was detected by using different commercial ELISA kits.</p><p><b>RESULTS</b>The absorbance value of pSS1adr-126 Asn and pSS1adr-126Ser plasmids were similar to that of the wild type HBsAg, the absorbance value of pSS1adw2-145Arg plasmids was lower than that of the wild type HBsAg.</p><p><b>CONCLUSION</b>It is estimated that the antigenicity of HBsAg mainly depended on the amino acid sequence of "a" antigen determinant and its conformation, so 145 amino acid substitutions led to the change of conformation and the antigenicity of variant HBsAg was lower than that of the wild type.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Ensayo de Inmunoadsorción Enzimática
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Transfección
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Chlorocebus aethiops
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Proteínas del Envoltorio Viral
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Medios de Cultivo Condicionados
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Células COS
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Alergia e Inmunología
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Vectores Genéticos
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Genética
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Antígenos de Superficie de la Hepatitis B
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2008
Tipo del documento:
Article