Genotype 1 JEV was isolated again from Liaoning Province, China, 2007 / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 91-94, 2008.
Article
en Zh
| WPRIM
| ID: wpr-254134
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To isolate Japanese encephalitis virus (JEV) from mosquitoes collected in Liaoning province and analysis the genotype of new isolated JEV strains and the characters of nucleotide and amino acid in the E gene.</p><p><b>METHODS</b>Collected mosquitoes in Dandong Liaoning Province in August, 2006. Virus isolation was using issue culture cells. Isolated viruses were identified by using serological and molecular methods.</p><p><b>RESULTS</b>Two new JEV strains, LNDG07-02 and LNDG07-16, were isolated from 1500 mosquitoes were belonging to genotype 1. The identity of nucleotide and amino acid of E gene between new JEV strains and live attenuated vaccine strain SA14-14-2 were 87.8-88% and 97.2%, respectively. Total 11 amino acid sites were differences in E gene between new isolates and SA14-14-2. However, there were no differentiation between the new JEV strains and the isolates in Donggang 2002.</p><p><b>CONCLUSION</b>Genotype 1 JEV was isolated again from Donggang, since the first isolation of this genotype in 2002. Genotype 1 JEV continues in existence in Donggang Liaoning Province.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Patología
/
Filogenia
/
Virología
/
Encéfalo
/
ARN Viral
/
Datos de Secuencia Molecular
/
Línea Celular
/
China
/
Epidemiología
/
Secuencia de Aminoácidos
Límite:
Animals
País/Región como asunto:
Asia
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2008
Tipo del documento:
Article