Effect of hepatitis B virus C protein on function of natural killer cell in NK-92 cells / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 275-277, 2008.
Article
en Zh
| WPRIM
| ID: wpr-254083
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the influence of hepatitis B virus C protein on the function of natural killer cell.</p><p><b>METHODS</b>Recombinant eukaryotic expression plasmid pHBI-CMV-HBC was constructed and confirmed by double restrictive enzyme digestion and DNA sequencing analysis. Then the recombinant plasmid was transfected into NK-92 cells with lipofectamine encapsuled. The transfected NK-92 cells containing expressive HBV C protein was confirmed by Western Blot analysis. ELISA was employed to determine the IFN-gamma level secreted by NK-92 cells. And finally the cytotoxicities of NK cells were analysed by MTT colorimetry, with the hepatoblastoma cell line (HepG2) as target cell.</p><p><b>RESULTS</b>Western blotting confirmed the expression of HBV C protein in the NK-92 cells transfected with pHBI-CMV-HBC. NK cytotoxicities and IFN-gamma secretion level of NK-92 cells transfected with recombinant plasmid significantly increased compared to control NK-92 cells transfected with blank plasmid (P < 0.01) and untransfected NK-92 cells(P < 0.01).</p><p><b>CONCLUSION</b>Transient expression of HBC can increase IFN-gamma secretion and cytotoxicities of NK-92 cells.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Virología
/
Células Asesinas Naturales
/
Línea Celular
/
Virus de la Hepatitis B
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Interferón gamma
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Citotoxicidad Inmunológica
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Alergia e Inmunología
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Genética
/
Hepatitis B
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Antígenos del Núcleo de la Hepatitis B
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2008
Tipo del documento:
Article