Expression and distribution of cysteinyl leukotriene receptors CysLT1R and CysLT2R, and GPR17 in brain of Parkinson disease model mice / 浙江大学学报·医学版
Zhejiang Daxue xuebao. Yixue ban
; (6): 52-60, 2013.
Article
en Zh
| WPRIM
| ID: wpr-252665
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To examine the spatiotemporal profiles and localization of CysLT1R, CysLT2R and GPR17 in mice with 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP)-induced Parkinson disease (PD).</p><p><b>METHODS</b>PD model was induced by subcutaneous injection of MPTP (25 mg/kg) for 5 d in adult male C57BL/6 mice. At d10 after MPTP injection, the expression and cellular localization of CysLT1R, CysLT2R and GPR17 in the substantia nigra were detected by immunohistochemistry and immunofluorescence.</p><p><b>RESULTS</b>CysLT1R, CysLT22 and GPR17 were normally localized in TH-positive dopaminergic neurons and microglia, while CysLT2R was also expressed in astrocytes. In dopaminergic neurons, approximately 91% co-expressed GPR17, 77% co-expressed CysLT1R and 52% co-expressed CysLT2R. Compared with the control group, TH-positive cells in the substantia nigra were significantly reduced in PD mice. CysLT1R, CysLT2R and GPR17-positive cells were significantly reduced; and CysLT1R, CysLT2R, GPR17-positive dopaminergic neurons were also significantly reduced in the PD group. In the striatum, both CysLT1R and GPR17 were normally expressed in neurons; whereas CysLT2R was expressed in astrocytes. In PD striatum, CysLT1R and GPR17-positive cells were decreased, but CysLT2R expression was significantly increased which mainly expressed in the proliferating astrocytes.</p><p><b>CONCLUSION</b>CysLT1R, CysLT2R and GPR17 may be involved in the MPTP-induced PD damage in mice.</p>
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Base de datos:
WPRIM
Asunto principal:
Enfermedad de Parkinson
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Encéfalo
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Receptores de Leucotrienos
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Receptores Acoplados a Proteínas G
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Modelos Animales de Enfermedad
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Metabolismo
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Ratones Endogámicos C57BL
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Proteínas del Tejido Nervioso
Límite:
Animals
Idioma:
Zh
Revista:
Zhejiang Daxue xuebao. Yixue ban
Año:
2013
Tipo del documento:
Article