Effects of angiotensin II on connexin 43 of VSMCs in arteriosclerosis / 浙江大学学报（英文版）（B辑：生物医学和生物技术）
J. Zhejiang Univ., Sci. B (Internet)
; (12): 648-653, 2006.
Article
en En
| WPRIM
| ID: wpr-251875
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of angiotensin II (Ang II) on expression of gap junction channel protein connexin 43 (Cx43) in the proliferation process of vascular smooth muscle cells (VSMCs) during the early stage of arteriosclerosis.</p><p><b>METHODS</b>Thirty-two adult male rabbits were randomly divided into 4 groups. Rabbits in Group A were fed common diet while others in Groups B, C, and D were fed high-cholesterol diet. Losartan (10 mg/(kg.d)) and ramipril (0.5 mg/(kg.d)) were added in the diet of Groups C and D, respectively. The animals were sacrificed after 8 weeks and abdominal aortas were removed and dissected. The expression of Cx43 was assayed using RT-PCR and Western Blotting analysis.</p><p><b>RESULTS</b>Cx43 was increased markedly in both protein and mRNA level in Groups B, C, and D fed high-cholesterol diet compared with that in control group (P<0.01). Cx43 level in losartan or ramipril treated groups was higher than that in control group (P<0.01, P<0.05), but lower than that in high-cholesterol diet groups (P<0.05, P<0.01).</p><p><b>CONCLUSION</b>Cx43 level was upregulated in VSMCs during early atherosclerosis. Losartan and ramipril can inhibit the expression of Cx43.</p>
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Base de datos:
WPRIM
Asunto principal:
Farmacología
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Fisiología
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Arteriosclerosis
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Sangre
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Peso Corporal
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ARN Mensajero
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Angiotensina II
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Colesterol
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Ramipril
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Conexina 43
Límite:
Animals
Idioma:
En
Revista:
J. Zhejiang Univ., Sci. B (Internet)
Año:
2006
Tipo del documento:
Article