Salidroside attenuates LPS-stimulated activation of THP-1 cell-derived macrophages through down-regulation of MAPK/NF-kB signaling pathways / 华中科技大学学报（医学）（英德文版）
J. huazhong univ. sci. tech. med. sci
; (6): 463-469, 2013.
Article
en En
| WPRIM
| ID: wpr-251447
Biblioteca responsable:
WPRO
ABSTRACT
Excessive activation of macrophages is implicated in various inflammatory injuries. Salidroside (Sal), one of the main bioactive components of Rhodiola Sachalinensis, has been reported to possess anti-inflammatory activities. This study aimed to examine the effect of Sal on the activation of macrophages and the possible mechanism. The lipopolysaccharide (LPS)-stimulated phrobol 12-myristate 13-acetate (PMA)-differentiated THP-1 macrophage models were established. The changes in the inflammatory profiles of THP-1-derived macrophages were determined. The results showed that Sal significantly decreased the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX2), interleukin-1beta (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) at both mRNA and protein levels in THP-1-derived macrophages, and the effect was dose-depedent. Moreover, NF-κB activation was significantly suppressed and the phosphorylation of ERK, p38 and JNK was substantially down-regulated after Sal treatment. The findings suggested that Sal can suppress the activation of LPS-stimulated PMA-differetiated THP-1 cells, as evidenced by the decreased expression of iNOS, COX2, IL-1β, IL-6 and TNF-α, and the mechanism involves the inhibition of NF-κB activation and the phosphorylation of the MAPK signal pathway.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Farmacología
/
Fenoles
/
Transducción de Señal
/
Regulación hacia Abajo
/
Línea Celular
/
Lipopolisacáridos
/
FN-kappa B
/
Proteínas Quinasas Activadas por Mitógenos
/
Alergia e Inmunología
/
Genética
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
J. huazhong univ. sci. tech. med. sci
Año:
2013
Tipo del documento:
Article