Splenectomy attenuates severe thermal trauma-induced intestinal barrier breakdown in rats / 华中科技大学学报（医学）（英德文版）
J. huazhong univ. sci. tech. med. sci
; (6): 868-873, 2015.
Article
en En
| WPRIM
| ID: wpr-250328
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WPRO
ABSTRACT
The severe local thermal trauma activates a number of systemic inflammatory mediators, such as TNF-α, NF-κB, resulting in a disruption of gut barrier. The gastrointestinal tight junction (TJ) is highly regulated by membrane-associated proteins including zonula occludens protein-1 (ZO-1) and occludin, which can be modulated by inflammatory cytokines. As splenectomy has been shown to reduce secretion of cytokines, we hypothesized that (1) severe scald injury up-regulates TNF-α and NF-κB, meanwhile down-regulates expression of ZO-1 and occludin, leading to the increased intestinal permeability, and (2) splenectomy can prevent the burn-induced decrease in ZO-1 and occludin expression, resulting in improved intestinal barrier. Wistar rats undergoing a 30% total body surface area (TBSA) thermal trauma were randomized to receive an accessorial splenectomy meanwhile or not. Intestinal injury was assessed by histological morphological analysis, and serum endotoxin levels, TNF-α, NF-κB, ZO-1 and occludin levels were detected by Western blotting in the terminal ileum mucosal tissue. 30% TBSA burn caused a significant increase in serum endotoxin levels, but NF-κB, and TNF-α, and the average intestinal villus height and mucosal thickness were decreased significantly. Burn injury could also markedly decrease the levels of ZO-1 and occludin in terminal ileum mucosal tissue (all P<0.01). Splenectomy at 7th day after burn significantly reversed the burn-induced breakdown of ZO-1 and occludin (all P<0.01). The results of this study suggest that severe thermal injury damages the intestinal mucosal barrier. Splenectomy may provide a therapeutic benefit in restoring burn-induced intestinal barrier by decreasing the release of inflammatory cytokines and recovering TJ proteins.
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Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Esplenectomía
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Sangre
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Western Blotting
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FN-kappa B
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Factor de Necrosis Tumoral alfa
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Ratas Wistar
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Endotoxinas
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Ocludina
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Proteína de la Zonula Occludens-1
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Calor
Límite:
Animals
Idioma:
En
Revista:
J. huazhong univ. sci. tech. med. sci
Año:
2015
Tipo del documento:
Article