Expression of Vitreoscilla hemoglobin and nitrilase in the yeast Pichia pastoris / 生物工程学报
Chinese Journal of Biotechnology
; (12): 730-735, 2004.
Article
en Zh
| WPRIM
| ID: wpr-249945
Biblioteca responsable:
WPRO
ABSTRACT
The expression of the vgb gene in vivo could improve the fermentation density and then contribute the extracellular secretion of the product of bxn gene. Constructed the recombination plasmid pPIC9K-vgbbxn and transformed into Pichia pastoris GS115. The results of PCR and SDS-PAGE indicate that the vgb gene and bxn gene had integrated into the genome of Pichia pastoris GS115 and expressed in efficient level. Also, the protein activity of their products had been verified respectively. Shake flask fermentation experiments showed that the presence of VHb in yeast Pichia pastoris efficiently enhanced cell growth and secretive expression of bxn gene under hypoxic habitats.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Fisiología
/
Pichia
/
Plásmidos
/
Proteínas Bacterianas
/
Proteínas Recombinantes
/
Reacción en Cadena de la Polimerasa
/
Electroforesis en Gel de Poliacrilamida
/
Hemoglobinas Truncadas
/
Genética
/
Aminohidrolasas
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Año:
2004
Tipo del documento:
Article