Spatio-temporal expression of the pathway-specific regulatory gene redD in S. coelicolor / 浙江大学学报（英文版）（B辑：生物医学和生物技术）
Journal of Zhejiang University. Science. B
; (12): 464-469, 2005.
Article
en En
| WPRIM
| ID: wpr-249188
Biblioteca responsable:
WPRO
ABSTRACT
Confocal laser scanning microscopy was used to observe the spatio-temporal expression of the pathway-specific gene redD during S. coelicolor cell cultivation. The corresponding mutant S. coelicolor lyqRY1522 carrying redD::eyfp in the chromosome was constructed. The temporal expression results of the fusion protein during submerged cultivation demonstrated that expression of redD began in the transition phase, continuing through the exponential growth phase to the stationary phase, and reached maximum in the stationary phase. On the other hand, redD was expressed only in substrate mycelia during solid-state culture, while aerial mycelia remained essentially non-fluorescent throughout culture. Results demonstrated that the expression pattern of redD coincides with that of the biosynthesis of the antibiotics during culture, revealing a direct correlation between the spatio-temporal distribution of regulatory gene expression and second metabolism.
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Base de datos:
WPRIM
Asunto principal:
Fisiología
/
Proteínas Bacterianas
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Transducción de Señal
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Regulación Bacteriana de la Expresión Génica
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Transactivadores
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Perfilación de la Expresión Génica
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Streptomyces coelicolor
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Genética
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Metabolismo
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Antibacterianos
Idioma:
En
Revista:
Journal of Zhejiang University. Science. B
Año:
2005
Tipo del documento:
Article