Determination of mangiferin in rat plasma and aqueous humor by HPLC / 中国中药杂志
Zhongguo Zhong Yao Za Zhi
; (24): 598-602, 2011.
Article
en Zh
| WPRIM
| ID: wpr-247424
Biblioteca responsable:
WPRO
ABSTRACT
A HPLC method was developed for the determination of mangiferin in rat plasma and aqueous humor. 4-Nitrophenol was used as internal standard. Analysis was performed on a Cosmosil ODS C18 analytical column (4.6 mm x 250 mm, 5 microm) with mobile phase consisting of methanol-water (40: 60) with 2% glacial acetic acid at a flow rate of 1.0 mL x min(-1). The calibration curve of mangiferin in rat plasma and aqueous humor showed excellent linear behaviors over the investigated concentration of 0. 50-250.00 mg x L(-1) in plasma and 0.10-10.00 mg x L(-1) in aqueous humor, respectively, and the correlation coefficients were all above 0.995 4. The intra-day and inter-day precisions for all samples were measured to be below 12%. The limit of quantitation was 0.10 mg x L(-1) and low enough for the determination of mangiferin in all samples. The validated method has been successfully applied to preliminary pharmacokinetics study of mangiferin in rat plasma and aqueous humor.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Humor Acuoso
/
Sangre
/
Farmacocinética
/
Química
/
Reproducibilidad de los Resultados
/
Cromatografía Líquida de Alta Presión
/
Sensibilidad y Especificidad
/
Ratas Wistar
/
Xantonas
/
Nitrofenoles
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Año:
2011
Tipo del documento:
Article