The Effects of Interferon/Retinoic Acid on Cervical Cancer Cell Lines According to the Mutational Status of HPV-URR / 대한암학회지
Journal of the Korean Cancer Association
; : 884-894, 2000.
Article
en Ko
| WPRIM
| ID: wpr-24520
Biblioteca responsable:
WPRO
ABSTRACT
PURPOSE: We investigated the effects of all-trans-retinoic acid (ATRA) and/or interferon-gamma (IFN-gamma) on the growth of various cervical cancer cell lines and HPV E6/E7 expression. The relationships between the functional activities of HPV-URR and the growth inhibition were identified. MATERIALS AND METHODS: Four groups of cell lines were included; i) with integrated form of HPV-16 DNA (SNU-17, CaSki), ii) episomal form of HPV-16 (SNU-523), iii) integrated form of HPV-18 (SNU-1160, HeLa) and iv) episomal form of HPV-18 (SNU-1245). The promoter activity of HPV-URR was confirmed by transient transfection assay in C33A using the HPV-18 URR-CAT reporter plasmid. RESULTS: Selective mutation was detected in TEF-1 (transcriptional enhancer factor) binding site in SNU-17, and the activity of URR in SNU-17 was higher than that of the prototype. The proliferation was more inhibited in SNU-17 by IFN-gamma (10 ng/ml) than in SNU-902, CaSki and HeLa. The increase of the HPV-URR activity might play a role in the inhibition of growth by interferon-g. The expression of HPV-16 E6/E7 were significantly decreased by ATRA or IFN-gamma. CONCLUSION: Point mutation at TEF-1 binding site of SNU-17 was related with the increased transcriptional activity of URR. Mutation in the HPV-URR and alteration of HPV-URR activity in SNU-17 might be related with significant growth suppression by IFN-gamma.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Plásmidos
/
Tretinoina
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Sitios de Unión
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ADN
/
Transfección
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Línea Celular
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Neoplasias del Cuello Uterino
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Interferón gamma
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Mutación Puntual
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Papillomavirus Humano 16
Tipo de estudio:
Prognostic_studies
Idioma:
Ko
Revista:
Journal of the Korean Cancer Association
Año:
2000
Tipo del documento:
Article