Apoptosis of retinoic acid resistant NB4-R1 cells induced with curcumin and its mechanism / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 340-343, 2010.
Article
en Zh
| WPRIM
| ID: wpr-243359
Biblioteca responsable:
WPRO
ABSTRACT
This study was purposed to explore the inhibitory effect of Curcumin on growth of retinoic acid-resistant acute promyelocytic leukemia (APL) cells and its mechanism. The NB4-R1, an APL cell line resistant to retinoic acid, was used as a model. The growth level of NB4-R1 was detected by MTT assay, the morphologic features of cells were observed by light microscopy, the mitochondrial transmembrane potential was determined by flow cytometry, the expressions of apoptosis-related proteins procaspase 3, caspase 3, PARP and BCL-XL were measured by Western blot. The results indicated that the sensitivity of NB4-R1 to Curcumin was consistent with NB4 though NB4-R1 was resistant to retinoic acid, Curcumin displayed inhibitory effect on growth of NB4-R1 in time-and concentration-dependent manners. The morphologic observation showed existence of apoptotic bodies in NB4-R1 cells treated with 20 micromol/L of Curcumin. The flow cytometry indicated that the mitochondrial transmembrane potential in NB4-R1 cells treated with 20 micromol/L of Curcumin obviously decreased. The Western blot detection revealed that expressions of pro-caspase 3 and BCL-XL were down-regulated, expressions of caspase 3 and sheared PAPP were up-regulated in NB4-R1 cells treated with 20 micromol/L of Curcumin. It is concluded that the Curcumin can inhibit the growth and induce the apoptosis of NB4-R1.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Farmacología
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Tretinoina
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Leucemia Promielocítica Aguda
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Poli(ADP-Ribosa) Polimerasas
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Apoptosis
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Curcumina
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Línea Celular Tumoral
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Proteína bcl-X
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Caspasa 3
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Poli(ADP-Ribosa) Polimerasa-1
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Año:
2010
Tipo del documento:
Article